Biological assessment of Berberis vulgaris and its active constituent, berberine: Antibacterial, antifungal and anti-hepatitis C virus (HCV) effect

Dynamic Chiropractic

Doaa A. Ghareeb¹  Eman E. M. Sarhan²  Abeer E. Abd El-Wahab³  Marwa M. Abu-Serie⁴ 

[1] Biochemistry Department, Faculty of Science, Alexandria University, Egypt.;City for Scientific Research and Technology Applications, P.O. Box 21934, Alexandria, Egypt.;Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, Egypt.;Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, Egypt.

关键词: Berberis vulgaris;    ethanolic extract;    gram positive bacteria;    gram negative bacteria;    anti-bacteria;    aflatoxines;    hepatitis C virus;    immune stimulation;    phagocytosis.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Berberis vulgarisis a shrub in the family Berberidaceae.B. vulgarisas well as other berberine (BER) containing plants are used medicinally in virtually all-traditional medical systems. In the present study, the efficacy of ethanolic extract of dried root powder ofB. vulgarisas anti bacterial, anti fungal and anti hepatitis C was evaluated. Also, its efficacy for induction of phagocytisis and lymphoporiferation was determined. The data showed thatB. vulgarisethanolic extract have strong anti bacterial effect (gram positive and gram negative) at concentrations range from 2.5 to 20 mg/ml. Anti fungal activity was measured according to inhibition of the production of aflatoxine B1 and B2, where ethanolic extract ofB. vulgariswas able to inhibit the production of 44 and 98.3% of aflatoxine B1 and 67.2 and 89% ofaflatoxine B2 at concentration of 0.01 to 0.1%, respectively. On the other hand, the ethanolic extract inhibits hepatitis C virus replication at concentration of 100 µg/ml, at single dose, while inducing lymphoproliferative effect and phagocytic activity in a dose dependant manner. In conclusion,B. vulgarisextract can be considered an efficient antimicrobial especiallyhepatitis C virus(HCV) andAspergillusflavus. This effect could be returned to its immunostimulant capacity as it enhances the activity ofperipheral blood mono nuclear cells(PMNC), especially phagocytic cells.

【 授权许可】

Unknown