Frontiers in Cellular and Infection Microbiology | |
The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae | |
Yuan, Fangyan1  Peng, Wei1  Liu, Ting1  Chen, Huanchun2  Cheng, Hui2  Li, Huan2  Yan, Kang2  Zhao, Haixu3  Chang, Peixi3  Liu, Feng3  Bei, Weicheng3  | |
[1] Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, China;State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, China;The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, China | |
关键词: Actinobacillus pleuropneumoniae; CpxA/CpxR; Biofilm; RpoE; Virulence; | |
DOI : 10.3389/fcimb.2018.00072 | |
学科分类:生物科学(综合) | |
来源: Frontiers | |
【 摘 要 】
Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia. In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae.
【 授权许可】
CC BY
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