期刊论文详细信息
Frontiers in Cellular and Infection Microbiology
The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
Yuan, Fangyan1  Peng, Wei1  Liu, Ting1  Chen, Huanchun2  Cheng, Hui2  Li, Huan2  Yan, Kang2  Zhao, Haixu3  Chang, Peixi3  Liu, Feng3  Bei, Weicheng3 
[1] Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agriculture, Huazhong Agricultural University, China;State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, China;The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, China
关键词: Actinobacillus pleuropneumoniae;    CpxA/CpxR;    Biofilm;    RpoE;    Virulence;   
DOI  :  10.3389/fcimb.2018.00072
学科分类:生物科学(综合)
来源: Frontiers
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【 摘 要 】

Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia. In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae.

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