Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Isolates between 2004 and 2014 in Yamagata, Japan: Change in Molecular Characteristics during an 11-year Period

Japanese journal of infectious diseases

Yoko Matsuzaki¹  Junji Seto¹  Tsutomu Itagaki¹  Seiji Hongo²  Yoshitaka Shimotai²  Yu Suzuki²  Katsumi Mizuta²  Fumio Katsushima³  Tatsuya Ikeda³  Yuriko Katsushima⁴ 

[1] Department of Infectious Diseases, Yamagata University Faculty of Medicine;Department of Microbiology, Yamagata Prefectural Institute of Public Health;Katsushima Pediatric Clinic;Yamanobe Pediatric Clinic

关键词: VNTR;    MLVA;    P1 type;    macrolide resistance;    molecular epidemiology;   

DOI  :  10.7883/yoken.JJID.2017.276

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

Multiple-locus variable-number tandem-repeat analysis (MLVA) typing was performed for Mycoplasma pneumoniae strains isolated between 2004 and 2014 in Yamagata, Japan. The results were examined by considering the combination of the P1 type and prevalence of macrolide resistance-associated mutations. Four-locus (Mpn13–16) MLVA classified 347 strains into 9 MLVA types, including 3 major types: 3-5-6-2, 4-5-7-2, and 4-5-7-3. All type 3-5-6-2 strains (77 strains) were P1 type 2 variants (2a or 2c), while types 4-5-7-2 (181 strains) and 4-5-7-3 (75 strains) were P1 type 1. MLVA type 4-5-7-2 strains circulated and were dominant until 2010, accounting for 88.4% of the 121 strains isolated between 2004 and 2010. The prevalence of types 4-5-7-3 and 3-5-6-2 strains increased rapidly in 2011 and 2012, respectively, resulting in cocirculation of 3 MLVA types, including type 4-5-7-2, between 2011 and 2013. The prevalence of macrolide resistance-associated mutations in MLVA types 4-5-7-2, 4-5-7-3, and 3-5-6-2 strains was 59.7% (108/181), 25.3% (19/75), and 0% (0/77), respectively. Because the prevalence of macrolide resistance-associated mutations differed by current MLVA types in Yamagata, continued surveillance combined with molecular typing and identification of macrolide resistance-associated mutations is necessary.

【 授权许可】

Unknown