MHC Class I Presentation and Regulation by IFN in Bony Fish Determined by Molecular Analysis of the Class I Locus in Grass Carp

The Journal of Immunology: Official Journal of the American Association of Immunologists

Yinan Jiang¹  George F. Gao¹  Li Wang¹  Zhenghu Jia¹  Changyou Lin¹  Xin Li²  Feng Gao²  Nianzhi Zhang²  Xiaoying Li²  Weihong Chen²  Chun Xia³  Ting Zhang³ 

[1] Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University;Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology and

DOI  :  10.4049/jimmunol.1000347

学科分类：生物科学（综合）

来源: American Association of Immunologists

【 摘 要 】

Beyond their sequences, little is known regarding MHC class I presentation and regulation by IFN in bony fish. In this work, the class I locus ( Ctid -UBA) was isolated from a grass carp fosmid library, and its polymorphisms and tissue expression were investigated. The Ctid -UBA and Ctid -β2–microglobulin genes then were expressed and refolded, and tetramer techniques were used to identify the CTL response. The interaction between grass carp type I IFN and Ctid -UBA genes was investigated. Two fosmids coding for Ctid -UBA *0101 and Ctid -UBA *0201 genes were sequenced. The SXY box and IFN-stimulated regulatory element motifs were located from the start codons to −800 bp in Ctid -UBA. A Southern blot showed three to four bands, suggesting that grass carp contains at least three class I loci. In addition, the Ctid -UBA allelic genes are expressed in all tissue of grass carp. The three-dimensional structure of Ctid -UBA *0102 showed that the peptide-binding domain was formed by the α1 and α2 domains, which could bind several nonapeptides of grass carp hemorrhagic virus. There were 1.60% more PE-positive cells in P1(QPNEAIRSL)-immunized fish than in blank and adjuvant control groups. Additionally, recombinant grass carp IFN could regulate the expression of Ctid- UBA. These results characterize the class I presentation, CTL response, and regulation by type I IFN in bony fish.

【 授权许可】

CC BY