Cytotoxic activities of Ferulago angulata extract on human leukemia and lymphoma cells by induction of apoptosis

Dynamic Chiropractic

Abbas Azadmehr¹  Samira Valiyari²  Fatemeh Zare Shahneh³  Reza Hajiaghaee⁴  Ali B⁵ 

[1] Department of Immunology, University of Medical Sciences Qazvin, Iran.;Drug Applied Research Center, Tabriz, Iran.;Immunology Research Center (IRC), Tabriz University of Medical Sciences, Tabriz, Iran.;Pharmacognosy and Pharmaceutics, Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.;Plant Breeding and Biotechnology Department, Faculty of Agriculture, University of Tabriz, Iran.

关键词: Ferulago angulata;    cytotoxic;    apoptosis;    antitumor;    lymphoma;    leukemia.;   

DOI  :  10.5897/JMPR12.923

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Ferulago angulata(FA), also known as Chovir, is one of the indigenous plants used in Persian medicinal herbs.Although, recent studies have demonstrated anti-tumor activities on cancer cellsin vitro, but theexact mechanism is not completely clarified. Furthermore, the objective of this study was to examine thein vitrocytotoxic activities of methanol extract ofF.angulataon B-celllymphoma (Raji), human leukemic monocyte lymphoma (U937), human acute myelocytic leukemia (KG-1A)and human umbilical vein endothelial (HUVEC)cell lines by a micro-culture tetrazolium test (MTT) cytotoxicity and trypan blue assays. Cell death enzyme linked immunosorbent assay (ELISA) was applied toquantify the nucleosome production subsequent nuclear DNA fragmentation during apoptosis and to test whether the mechanism involves induction of apoptosis or not. Results showed that methanol extract at 50 to 800 µg/ml time and dose-dependently suppressed the proliferation of Raji, U937 and KG-1A cells by more than 80% (p < 0.01), with ascending order of IC50values in 24 h: Raji (121.404 µg/ml), U937 (208.019 µg/ml) and KG-1A (211.011 µg/ml). The extract did not exertany significant cytotoxic effect on normal cell line HUVEC (IC50> 800 µg/ml). Nucleosome productions in Raji, U937 and KG-1A cells were significantly increased upon the treatment ofF.angulataextract.F.angulataextract was found to selectively time and dose-dependently inhibit theproliferation of lymphoma and leukemic cells probably via an apoptosis-dependent pathway.

【 授权许可】

Unknown