Comparative Evaluation of Serological and Molecular Methods for the Diagnosis of Scrub Typhus in Indian Settings

Japanese journal of infectious diseases

Vishnubhatla Sreenivas¹  Aparajit Ballay Dey²  Bijay Ranjan Mirdha³  Rama Chaudhry³  Nitin Gupta³  Bimal Kumar Das³  Sushil K Kabra⁴  Rakesh Lodha⁴ 

[1] Department of Biostatistics, All India Institute of Medical Sciences;Department of Geriatric Medicine, All India Institute of Medical Sciences;Department of Microbiology, All India Institute of Medical Sciences;Department of Pediatrics, All India Institute of Medical Sciences

关键词: Orientia tsutsugamushi;    scrub typhus;    immunofluorescence assay;   

DOI  :  10.7883/yoken.JJID.2016.139

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

The major brunt of scrub typhus is borne by developing countries like India but the disease remains neglected. The rapid progression of this infection to serious complications and associated mortality calls for improved diagnostics. The immunoglobulin (Ig)M immunofluorescence assay (IFA), with all of its limitations remains the gold standard. Of 218 suspected cases from patients enrolled in a 2-year study, 30 cases of scrub typhus were detected by IgM IFA, using a 1:64 dilution. The sensitivities of the IgM enzyme-linked immunosorbent assay, IgM rapid flow assay (RFA), and IgG RFA were found to be 97%, 87%, and 77%, respectively. Their respective specificities were 100%, 100%, and 92%, respectively. The sensitivity and specificity of a nested PCR assay targeting a gene encoding a 56-kDa protein were found to be 50% and 100%, respectively.

【 授权许可】

Unknown