Hairy root culture of Przewalskia tangutica for enhanced production of pharmaceutical tropane alkaloids

Dynamic Chiropractic

关键词: Przewalskia tangutica;    hairy root;    scopolamine;    hyoscyamine.;   

DOI  :  10.5897/JMPR09.289

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The bacteria-free seedlings ofPrzewalskia tanguticawere germinated from seeds. The leaves were used as initial explants for genetic transformation mediated byAgrobacterium rhizogenesstrain A4, with the induction of Acetosyringone. The hairy roots were induced from the wounded sites of the leaves at the frequency of 100%. The independently transformed and fast growing hairy roots were subcultured in MS medium to establish the hairy root culture lines. Genomic DNA PCR analysis was applied to detect the presence of the rooting genes, includingrolBandrolCthat had integrated into the genome ofPrzewalskia tangutica. After 5-week culture in MS liquid medium, the hairy roots produced scopolamine and hyoscyamine at higher levels by comparison to the wild-type roots. The best scopolamine-production line produced 0.68 mg/g DW scopolamine, and the best hyoscyamine-production line produced 1.13 mg/g DW hyoscyamine. It was the first time that hairy root cultures ofP. tanguticawere established to produce tropane alkaloids.

【 授权许可】

Unknown