Development of a Novel Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Rickettsia spp.

Japanese journal of infectious diseases

Shuji Ando¹  Masaaki Satoh¹  Mutsunori Shirai²  Nozomu Hanaoka²  Motohiko Ogawa²  Minenosuke Matsutani³ 

[1] Department of Microbiology and Immunology, Yamaguchi University School of Medicine;Department of Virology-1, National Institute of Infectious Diseases;Infectious Disease Surveillance Center, National Institute of Infectious Diseases

关键词: Rickettsia;    LAMP assay;    orthologous genes;   

DOI  :  10.7883/yoken.JJID.2015.579

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

We developed a novel loop-mediated isothermal amplification (LAMP) method to detect Rickettsia spp., including Rickettsia prowazekii and R. typhi. Species-specific LAMP primers were developed for orthologous genes conserved among Rickettsia spp. The selected modified primers could detect all the Rickettsia spp. tested. The LAMP method was successfully used to detect 100 DNA copies of Rickettsia spp. within approximately 60 min at 63℃. Therefore, this method may be an excellent tool for the early diagnosis of rickettsiosis in a laboratory or in the field.

【 授权许可】

Unknown