Comparison of Three Molecular Subtyping Methods among O157 and Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Japanese Cattle

Japanese journal of infectious diseases

Atsushi Iguchi¹  Jun Ogasawara²  Hiromi Nakamura²  Atsushi Fujiwara³  Kyohei Fujiwara³  Tomofumi Maehara³ 

[1] Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki;Microbiology Section, Osaka Institute of Public Health;Osaka City Meat Inspection Center

关键词: STEC;    beef cattle;    MLST;    MLVA;    PFGE;   

DOI  :  10.7883/yoken.JJID.2017.297

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

To determine the infection source, route, and extent of an outbreak, it is important to subtype Shiga toxin-producing Escherichia coli (STEC) isolates belonging to the same serotype for clustering into clonally related groups. In this study, we compared 3 molecular subtyping methods—multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and multiple-locus variable-number of tandem repeat analysis (MLVA)—using O157 and non-O157 STEC isolates from Japanese beef cattle. A total of 73 STEC isolates belonging to 9 O-serogroups were analyzed. By means of 3 molecular subtyping methods, the strains were subdivided into 9 MLST sequence types (STs), 23 PFGE types, and 26 MLVA types. The STEC classification by O-serogrouping and MLST was almost identical. Furthermore, PFGE and MLVA could systematically classify STEC isolates of the same serotypes and STs. MLVA and PFGE were found to be highly efficient subtyping methods after O-serogrouping for the classification of not only O157 but also non-O157 STEC isolates in an outbreak investigation.

【 授权许可】

Unknown