Systematic Identification of Intracellular-Translocated Candidate Effectors in Edwardsiella piscicida

Frontiers in Cellular and Infection Microbiology

Liu, Qin¹  Zhang, Yuanxing²  Yang, Dahai³  Zhang, Lingzhi³  Jiang, Zhiwei³  Wang, Qiyao³  Huang, Yajun³  Fang, Shan³ 

[1]Shanghai Collaborative Innovation Center for Bio-Manufacturing Technology, China

[2]Shanghai Engineering Research Center of Maricultured Animal Vaccines, China

[3]State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, China

关键词: effector;    Edwardsiella piscicida;    T3SS;    T6SS;    OMV;   

DOI  :  10.3389/fcimb.2018.00037

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Many bacterial pathogens inject effectors directly into host cells to target a variety of host cellular processes and promote bacterial dissemination and survival. Identifying the bacterial effectors and elucidating their functions are central to understanding the molecular pathogenesis of these pathogens. Edwardsiella piscicida is a pathogen with a wide host range, and very few of its effectors have been identified to date. Here, based on the genes significantly regulated by macrophage infection, we identified 25 intracellular translocation-positive candidate effectors, including all five previously reported effectors, namely EseG, EseJ, EseH, EseK and EvpP. A subsequent secretion analysis revealed diverse secretion patterns for the 25 effector candidates, suggesting that multiple transport pathways were involved in the internalization of these candidate effectors. Further, we identified two novel type VI secretion system (T6SS) putative effectors and three outer membrane vesicles (OMV)-dependent putative effectors among the candidate effectors described above, and further analyzed their contribution to bacterial virulence in a zebrafish model. This work demonstrates an effective approach for screening bacterial effectors and expands the effectors repertoire in E. piscicida.

【 授权许可】

CC BY