Doubling the chromosome number of Salvia hains using colchicine: Evaluation of morphological traits of recovered plants

Dynamic Chiropractic

Heidar Meftahizade¹  Vahid Rahimi²  Naser Lotfi³  Mohammad Sadat Hosseini Grouh⁴ 

[1] ACECR Medicinal Plants Research Center, Ilam, Iran.;Department of Horticulture, Abouraihan Campus, University of Tehran, Tehran, Iran.;Department of Medicinal Plants, College of Miandoab, University of Uremia, Uremia, Iran.;Department of Plant Sciences, Faculty of Agriculture, University of Jiroft, Jiroft, Iran.

关键词: Autotetrapolid;    flowcytometry;    polyploid;    Salvia hains;    chromosome counting.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

This study demonstrates a protocol of polyploidy induction inSalvia hains, in order obtained tetraploid five concentrations (0.1, 0.3, 0.4, 0.5 and 0.7%) of colchicine were used to determine the best treatment for the induction of tetraploid plants. Treatment seeds with colchicine for 24 h product tetraploid plants. Ploidy level of the resulting plantlets was determined by morphological study, chromosome counting and flowcytometry. Tetraploid plants were obtained fromS. hainsafter applied concentrations 0.3, 0.4 and 0.5% of colchicine. Plants obtained from 0.1% colchicinewere all diploids. The induced tetraploid in S. hains was accompanied by larger stomata increase in chloroplast number in guard cells and decrease in stomata density, compared to diploid (control) plants. Thetechniques used to induce tetraploidS. hainsplants by colchicine were successful and could be used in breeding programs.

【 授权许可】

Unknown