Reduction of small arteries contractility with improving the relaxation properties by Ginkgo biloba extract

Dynamic Chiropractic

Ruta Masteikova¹  Genuvaite Civinskiene²  Saule Velziene³  Egle Zelbiene⁴  Serpil Cecen⁵  Ausra Burkauskienė⁶  Ale Laukeviciene⁷ 

[1] Adnan Menderes University, Aydin, Turkey.;Department of Drugs Technology and Social Pharmacy, Lithuanian University of Health Sciences, Kaunas, Lithuania.;Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.;Department of Physiology, Institute of Anatomy, Lithuannian University of Health Sciences, Kaunas, Lithuania.;Institute of Physiology and Pharmacology, Lithuanian University of Health Sciences, Kaunas, Lithuania.

关键词: Ginkgo biloba;    myography;    contractility;    endothelium;    vascular smooth muscles.;   

DOI  :  10.5897/JMPR11.1704

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The effect of ethanolicGinkgo bilobaL. leaf extract (GE) on vascular smooth muscle contractility and endothelium-dependent relaxation were investigated in this study. Direct applications on isolated vessels from Wistar rats as well as animal feeding with the extract (dosage of 0.32 ml/kg for 10 days) were used. Vascular contractility of small isolated mesenteric arteries of the animals was examined using wire myography. The extract significantly suppressed the vascular contraction to KCl in dose-dependent mode (4´ 10-3mg/ml of phenolic compounds (PC) – up to 82%; 4 ´10-2mg/ml of PC – 37%, p < 0.01; and 0.4 mg/ml of PC – 4%, p < 0.001). Ten (10) days of administration of the extract for the animals in drinking water resulted in slight decrease of contractility to KCl (95%) and phenylephrine (88%). Both the endothelium-dependent relaxation to acetylcholine and endothelium-independent relaxation to sodium nitroprusside (SNP) were slightly improved by shifting the dose–response curve leftward with the significantly improved maximum relaxation (86.39 ± 3.61 to 60.84 ± 12.45%, p < 0.05 with 1 mM acetylcholine and 82.86 ± 8 to 55.86 ± 10.85%; p < 0.05, with 1 mM SNP). These results suggested that GE improved and revealed the vasorelaxant effects mainly attributed to smooth muscle involving mechanisms without impairment of the endothelium-depended relaxation.

【 授权许可】

Unknown