Phytochemical and antimicrobial studies of the ethyl acetate extract of Alchornea cordifolia leaf found in Abuja, Nigeria

Dynamic Chiropractic

Josiah Ademola Onaolapo¹  Gbonjubola Olusesan Adeshina² 

[1] Department of Microbiology, Kogi State University, Anyigba, Kogi State, Nigeria.;Department of Pharmaceutics and Pharmaceutical Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.

关键词: Alchornea cordifolia;    ethyl acetate extract;    phytochemical;    antimicrobial activity;    fractionation.;   

DOI  :  10.5897/JMPR09.315

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The phytochemical screening of the ethyl acetate extract revealed the presence of tannins, flavonoids, glycosides, resins and carbohydrates. Seventy-eight fractions were elucidated from the extract using the bioassay-guided fractionation by employing the accelerated gradient chromatography (AGC) technique. All the fractions showed antimicrobial activity against the test organisms. Antimicrobial activities of the ethyl acetate extract ofAlchornea cordifolialeaf againstPseudomonas aeruginosa, Staphylococcus aureus, Escherichia coliandCandida albicans(clinical isolates);Ps. aeruginosaATCC 10145, S. aureusATCC 12600, E. coliATCC 11775and C. albicansATCC 18804 using agar well diffusion and agar diffusion methods showed that the extract possess broad spectrum of activity against the test Gram-negative and Gram-positive bacteria and the fungi/yeast with diameter of zones of inhibition ranging from 10.0 - 35.0 mm. The minimum inhibitory concentration (MIC.) and the minimum bactericidal concentration (MBC) of the extract against the test organisms were found to fall between 0.625 - 10 mg/ml. The results of the rates of kill revealed that ethyl acetate extract produced 100% kill ofPs. aeruginosa, E. coliandS. aureusat low concentrations after 2 h. The results have therefore proved the claims of traditional healers in the use of the plant leaf.

【 授权许可】

Unknown