In vitro development of embryogenic calli and embryogenic stages in suspension cultures of mangosteen (Garcinia mangostana L.)

Dynamic Chiropractic

Innaka Ageng Rineksane¹  Saleh Kadzimin²  Mihdzar Abdul Kadir³ 

[1] Department of Agriculture Technology, Faculty of Agriculture, University Muhammadiyah Yogyakarta, Indonesia.;Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Seri Kembangan, Selangor, Malaysia.;Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Seri Kembangan, Selangor, Malaysia.

关键词: Mangosteen;    embryogenic callus;    suspension culture;    in vitro.;   

DOI  :  10.5897/JMPR11.197

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

In this study, the potential of uncoated and coated mangosteen seed explants in forming embryogenic callus were examined in the basal Linsmaier and Skoog medium supplemented with different auxins at various concentrations. Among the highest percentage of callus response (93.3%) was obtained when uncoated seed explants were cultured in basal LS medium containing 8 mg/L 2,4-D. Combining of cytokinins and 2,4-D to improve embryogenicity of calli showed that among the highest percentage of callus response (80%) and the lowest percentage of callus browning (53.53%) with yellowish and compact nodular calli were obtained on MS medum supplemented with 8 mg/L 2,4-D and 0.1 mg/L BAP. Addition of glutamine into MS medium containing 8 and 0.1 mg/L BAP performed that glutamine did not increase the growth of calli, however texture (friable) and color callus (yellowish) was improved. The growth and multiplication of cells in suspension cultures showed that the cells were able to divide and proliferate even though cultured in half strength MS liquid medium without 2,4-D. After six months of culture, the heart embryogenic stage was obtained only on medium supplemented with 1 mg/L BAP. The globular and torpedo embryogenic stages were obtained on media supplemented with 1, 3 and 9 mg/L TDZ after five months of culture.

【 授权许可】

Unknown