Investigation of the effectiveness of ghrelin treatment in lung tissue of rats with sepsis

Bratislava Medical Journal

H. Yorulmaz¹ 

关键词: apoptosis;    cytokine;    ghrelin;    lipopolysaccharide;   

DOI  :  10.4149/BLL_2017_112

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

OBJECTIVE: We aimed to investigate the effects of exogenous ghrelin on cytokine and ghrelin levels, oxidant parameters, and apoptotic genes in lung tissue during sepsis. BACKGROUND: There was evidence that changes of apoptosis are linked with morbidity and mortality in sepsis. There were scarce studies on the effect of ghrelin on apoptotic genes and endogenous ghrelin levels during sepsis.METHODS: Male Wistar albino rats 200–250 g were separated into four groups; Control, LPS (5 mg/kg), Ghrelin (10 nmol/kg i.v.), and LPS+Ghrelin. Tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), and ghrelin levels were determined from lung tissue using enzyme-linked immunosorbent assay (ELISA). TNF-α, IL-10, Bcl-2, and Bax gene expressions were calculated using quantitative real-time polymerase chain reaction (RT-PCR), tissue superoxide dismutase enzyme (SOD) activities and malondialdehyde (MDA) were determined spectrophotometerically.RESULTS: TNF-α levels decreased in the LPS+Ghrelin group compared with the LPS (p < 0.001).

【 授权许可】

CC BY