In vitro and in vivo investigations of the wound healing effect of crude Spirulina extract and C-phycocyanin

Dynamic Chiropractic

Canan Sevimli Gur¹  Pergin Atilla²  Ilyas Onbasılar³  Nur Cakar⁴  Deniz Kiraz Erdogan⁵ 

[1] Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey.;Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, 06100 Ankara, Turkey.;Experimental Animal Research and Husbandry Unit, Faculty of Medicine, Hacettepe University, 06100 Ankara, Turkey.;Kocaeli University, Science and Literature Faculty, Department of Biology, Biotechnology Discipline, Kocaeli, Turkey.;Quintiles Diagnostic Researches, 34337 Istanbul, Turkey.

关键词: Spirulinaextract;    c-phycocyanin;    in vitro;    in vivo;    Sprague-Dawley rats;    3-(4;    5-Dimethylthiazol-2-yl)-2;    5-diphenyltetrazolium bromide (MTT);    tissue regeneration.;   

DOI  :  10.5897/JMPR12.705

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The aim of this study was to evaluate the influence of crudeSpirulinaextract and C-phycocyanin (C-PC) isolated from crudeSpirulinaextract on cultured human keratinocyte, usingin vitroandin vivomodels of wound healing.Spirulinahas been used as a nutraceutical and source of potential pharmaceuticals, however it is not known which component of the cyanobacteria is effective for wound healing. Inin vitromodel, cultured human keratinocyte were used to investigate the effects of crudeSpirulinaextract (PSE) and C-phycocyanin (C-PC) extracts on processes involved in keratinocyte proliferation, regeneration and migration. Keratinocyte proliferation and regeneration were monitored by the colorimetric (MTT) assay and migration was monitored in relation to the closure of a denuded area scratched in a confluent monolayer. On the other hand, inin vivomodel using Sprague-Dawley male rats, effects of PSE and C-PC on tissue regeneration were investigated. Results ofin vivowound healing study were monitored by means of histological examinations. PSE extract showed the best growth stimulation at 33.5 µg/mL dose of treatment, which revealed a cell viability ranging from 100 to 270% after 72 h. Cell viability was also good for C-PC and was measured as high as 213%. Cell viability and proliferation difference between PSE and C-PC were observed not to be significant (p > 0.05) at the range of doses (33.5 to 0.0335 µg/mL) studied. Inin vivoefficiency of the PSE and C–PC, it was observed that 1.25% C-PC has a better effect on the 7th day compared to other preparations.

【 授权许可】

Unknown