Effect of acid hydrolysis in the microwave-assisted extraction of phenolic compounds from Geranium sibiricum Linne with the guidance of antibacterial activity

Dynamic Chiropractic

Ji Li¹  Yu-Chun Yang#²  Zhong-Hui Zhang³  Zhi-Wei Yang#⁴ 

[1] Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China.;Forestry Academy of Jilin province, Changchun 130033, China.;Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.;School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China.

关键词: Phenols;    Hydrolysis method;    Microwave-assisted extraction;    Geranium sibiricumLinne;    Antibacterial activity.;   

DOI  :  10.5897/JMPR12.154

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The feasibility and the effect of acid or alkali hydrolysis in microwave-assisted extraction as well as the antibacterial activity of the resulting extracts were studied to select appropriate phenols extraction method.The results demonstrated that acid hydrolysis was suitable for the extraction of phenolsfromGeranium sibiricumand hydrochloric acid (HCl) hydrolysis do not only performed the highest effectiveness, but also enhanced its antibacterial capacity. After the process was optimized, the total phenols content, yields of corilagin and geraniin were increased by 28.60, 53.80 and 35.65%, respectively, as compared to only 20% inmicrowave-assisted extraction (MAE). Scanning electron microscopy (SEM) was employed to verify the destructive effects of the methods, the results represented that HCl hydrolysis in MAE methodhad dramatic destructiveness on cell wall.Thus,HCl hydrolysis in MAEprovides an efficient and rapid approach forthe natural products extraction and the research alsoprovides a valuable nature resource for healthy food industry.

【 授权许可】

Unknown