Anti-proliferation and anti-migration activities of ten selected Zingiberaceae species against MDA-MB-231 cells

Dynamic Chiropractic

Nurhayati Zainal Abidin¹  Ann Jee Chew² 

[1] Center for Foundation Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia;Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

关键词: Zingiberaceae;    anti-metastatic activity;    anti-migration;    anti-proliferation;    MDA-MB-231;    selectivity index.;   

DOI  :  10.5897/JMPR11.599

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Zingiberaceaeplants exhibit various biological activities, but scientific knowledge of the plants in anti-metastatic aspects is still very limited. The objective of this study is to examine the anti-metastatic potentials of 30 crude extracts from ten selected localZingiberaceaespecies on hormone-independent, highly metastatic human breast cancer cells, MDA-MB-231. TheZingiberaceaerhizomes were extracted with petroleum ether, chloroform, and methanol using Soxhlet extractor system. Effects of the 30 extracts on proliferation and migration of MDA-MB-231 cells were evaluated using3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay and scratch wound assay, respectively. Besides cytotoxicity, it is also vital to determine the potential toxicity of the extract in order to select the most promising extracts for anti-cancer drugs development. Thus, a special parameter–Selectivity Index (SX) was utilized as the selectivity indicator of tested extracts towards tumour cells. The results revealed thatpetroleum ether extracts ofAlpinia galanga,Boesenbergia rotunda, andZingiber zerumbet; as well as chloroform extracts ofAlpinia galanga,Boesenbergia rotunda, andCurcuma domesticawere screened to possess the most effective anti-proliferation and anti-migration activities against MDA-MB-231 cells. Three most desirable extracts were selected based on both their anti-metastatic potentials and SX, and were subjected for qualitative analysis using thin-layer chromatography (TLC).

【 授权许可】

Unknown