Antifungal activity of coptidis rhizoma against Candida species

Dynamic Chiropractic

关键词: Anticandidial activity;    coptidis rhizoma;    MTT assay;    HaCaT cell line.;   

DOI  :  10.5897/JMPR11.272

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The extract of Coptidis Rhizoma was determined antifungal activity against five differentCandidaspecies. The extract showed antifungal activity againstCandida albicansat 200 μg/ml, andC. tropicalisandC. glabrataat 50 μg/ml. A compound 1 was separated by thin layer chromatography (TLC) analysis and confirmed as berberine by high performance liquid chromatography (HPLC) analysis. The minimum inhibitory concentration (MIC) values of the compound 1 and berberine were 24 and 48 μg/ml againstC. tropicalisandC. glabrata, and 30 and 50 μg/ml againstC. tropicalisandC. glabrata. The IC50values of the compound 1 were 58 μM at 24 h and 40 μM at 48 h by MTT assay of cell viability using HaCaT cell line. The above results indicated that berberine was a good candidate to inhibit the propagation ofC. tropicalisandC. glabrata, even in human skin.

【 授权许可】

Unknown