Analysis of anti-oxidant activity of medicinal plants according to the extracted parts

Dynamic Chiropractic

Hyun-Jung Jo¹  Yu-Su Shin² 

[1] Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 609-757, Republic of Korea.;Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Chungbuk 369-873, Republic of Korea.

关键词: Anti-oxidants;    1;    1-diphenyl-2-picrylhydrazyl (DPPH);    3-[4;    5-dimethylthiazol-2-yl]-2;    5-diphenyltetrazolium bromide (MTT);    lactate dehydrogenase(LDH) cytotoxicity;    Oenothera odorata.;   

DOI  :  10.5897/JMPR11.789

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

In this research, we compared and analyzed the anti-oxidant activity of ten medicinal plants using an oriental medicine and a folk remedy. Among them, the extracts fromOenothera odoratahad the highest anti-oxidant effect. The extracts from the root, stem and flower ofO. odoratawere tested by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test, 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) cell viability assay, andlactate dehydrogenase(LDH) cytotoxicity assay. Stem and flower extracts ofO. odoratawere similar to the activity of quercetin, one of the most anti-oxidants in DPPH radical scavenging activity test, and the root extracts showed a weak DPPH radical scavenging activity. In MTT cell viability assay, the extracts from the flower, stem, and root were resistant against hydrogen peroxide (H2O2) treatment in order. The extracts from the root, stem and flower showed higher cell protection effect than those from quercetin against LDH cytotoxicity. And β-sitosterol from the extracts of stem was isolated. These results suggest that the extracts from the flower, stem, and root ofO. odoratamight be a source of anti-oxidants.

【 授权许可】

Unknown