Journal of Functional Morphology and Kinesiology | |
Image-Based Histological Evaluation of Scaffold-Free 3D Osteoblast Cultures | |
Eggert, Sebastian1  | |
关键词: bone tissue engineering; 3D cell culture; histomorphometry; open-source software; image-based analysis; ImageJ; | |
DOI : 10.3390/jfmk2040042 | |
学科分类:力学,机械学 | |
来源: mdpi | |
【 摘 要 】
The analysis of tissue network characteristics and cell distribution using histological methods is widely used. However, image analysis still relies on manual evaluation methods, known as semi-quantitative analysis, which are time-consuming and to a certain degree user-specific. For this reason, automated imaging processing methods have an enormous potential to increase sample processing and reduce the variation that is caused by a user-specific evaluation. This work demonstrates the feasibility of using a semi-automated image analysis process based on the open source software framework ImageJ and the plug-in Angiogenesis Analyzer to evaluate the quantitative degree of tissue damage within 3D cell constructs after mechanical loading. Within a proof-of-concept study, the semi-automated approach was applied to calculate the Node-to-Free Ratio (N/F-Ratio) and perform a strut analysis for histological evaluation of mechanically compressed samples of human osteoblast-derived 3D constructs. The N/F-Ratio revealed a median value of 1.29 for the control, whereas the values for the mechanically compressed samples decreased to 0.97 for 20% compression, 0.85 for 40%, and 0.86 for 60%. The strut analysis indicated a decrease of the connected branches with increasing compression rate. The newly developed and time-saving processing workflow was successfully established and can be carried out using available, open source software solutions.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO201902022149136ZK.pdf | 6986KB | download |