Assessment of genetic diversity in two Allium spp. using random amplified polymorphic DNA markers

Dynamic Chiropractic

关键词: Alliumcepa;    Allium ascalonicum;    genetic diversity;    Nigeria;    random amplified polymorphic DNA(RAPD).;   

DOI  :  10.5897/JMPR12.373

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Genetic diversity was estimated among fifteen local cultivars of twoAlliumspecies (14 ofAlliumcepaand 1Allium ascalonicum) using 6 random amplified polymorphic DNA (RAPD) primers. A total of 54 RAPD amplicons were obtained, of which 25 (46.3%) were polymorphic. The percent polymorphism between primers ranged from 6.9 to 30.2%. The highest genetic distance (75%) was observed between light brown and white phenotypes.A. ascalonicumproduced a distinct banding pattern from all other 14 genotypes belonging toA. cepa.Genetic diversity within and between the four phenotypic (colour) classes showed that highest variability exists within the purple cultivars. Nei’s gene diversity ranged from 0.11 ± 0.19 in the light brown phenotypic groups to 0.27 ± 0.18 in the purple group. The observed number of alleles was 1.79 ± 0.41 and effective number of alleles was 1.46±0.35 for the purple phenotype, while 1.27±0.45 and 0.19±0.32 alleles represent the observed alleles and effective number of alleles in detecting polymorphism the light brown phenotype, respectively. Dendrogram constructed from similarity coefficient produced three clusters. The On8 and On10 cultivars from Borno and Kebbi states respectively were the closest probably as a result of exchange of genetic materials between farmers and for commercial purpose being an open pollinated crop, while On16 and On17 clustering together may be due to collection from same location and adaptation to the environment. Results are discussed in the light of current taxonomic delineations which considerA. ascalonicumas a subspecies ofA. cepa. Interspecific Hybridization betweenA. cepaandA. ascalonicumis encouraged in order to introduce medicinally active compounds like flavonoids intoA. cepa.

【 授权许可】

Unknown