Anaplasma phagocytophilum MSP4 and HSP70 Proteins Are Involved in Interactions with Host Cells during Pathogen Infection

Frontiers in Cellular and Infection Microbiology

de la Fuente, José¹  Gortazar, Christian²  s, James J.³  rez, Ana L.⁵  Ferná⁵  Contreras, Marinela⁵  Aylló⁵  Villar, Margarita⁷  a-Pé⁷  ndez, Lourdes⁷  Valdé⁷  Alberdi, Pilar⁸  ndez de Mera, Isabel G.⁸  n, Nieves⁸  Vancová⁸  Stuen, Snorre⁹  Mateos-Herná^1,10  Garcí^1,10  Cabezas-Cruz, Alejandro^1,10  , Marie^1,10 

[1] Budějovice, Czechia;Biology Centre, Czech Academy of Sciences, Institute of Parasitology, ČeskéDepartamento de Sanidad Animal, Instituto Vasco de InvestigacióFaculty of Science, University of South Bohemia, ČeskéSaBio, Instituto de InvestigacióUMR BIPAR, Animal Health Laboratory, INRA, ANSES, ENVA, Maisons Alfort, France;ficas, CSIC-UCLM-JCCM, Ciudad Real, Spain;n en Recursos Cinegén y Desarrollo Agrario (NEIKER), Derio, Spain;ticos, Consejo Superior de Investigaciones Cientí

关键词: Anaplasmosis;    immunology;    HL60;    Ticks;    Vaccines;    Sheep;    Anaplasma phagocytophilum;   

DOI  :  10.3389/fcimb.2017.00307

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Anaplasma phagocytophilum transmembrane and surface proteins play a role during infection and multiplication in host neutrophils and tick vector cells. Recently, A. phagocytophilum Major surface protein 4 (MSP4) and Heat shock protein 70 (HSP70) were shown to be localized on the bacterial membrane, with a possible role during pathogen infection in ticks. In this study, we hypothesized that A. phagocytophilum MSP4 and HSP70 have similar functions in tick-pathogen and host-pathogen interactions. To address this hypothesis, herein we characterized the role of these bacterial proteins in interaction and infection of vertebrate host cells. The results showed that A. phagocytophilum MSP4 and HSP70 are involved in host-pathogen interactions, with a role for HSP70 during pathogen infection. The analysis of the potential protective capacity of MSP4 and MSP4-HSP70 antigens in immunized sheep showed that MSP4-HSP70 was only partially protective against pathogen infection. This limited protection may be associated with several factors, including the recognition of non-protective epitopes by IgG in immunized lambs. Nevertheless, these antigens may be combined with other candidate protective antigens for the development of vaccines for the control of human and animal granulocytic anaplasmosis. Focusing on the characterization of host protective immune mechanisms and protein-protein interactions at the host-pathogen interface may lead to the discovery and design of new protective antigens.

【 授权许可】

CC BY