High performance liquid chromatography (HPLC) analysis of embelin in different samples of Embelis ribes Burm. f. - a threatened medicinal plant of India

Dynamic Chiropractic

A. Sabitha Rani¹  V. Nagamani²  M. Satyakala³ 

[1] Biology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.;Department of Botany, Osmania University College for Women, Koti, Hyderabad, India;Director, PharmaTrain, Hyderabad, India

关键词: Embelia ribes(Mysrinaceae);    medicinal plant;    seed extract;    high performance liquid chromatography (HPLC) analysis;    embelin.;   

DOI  :  10.5897/JMPR2013.4447

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Embelia ribes.Burm.f.is one of the important medicinal plants of India. Six samples ofEmbeliawere collected from different geographical regions of India. The seed extract was isolated and analyzed for the principle bioactive constituent, embelin, by high performance liquid chromatographic (HPLC)method. All the samples showed characteristic peaks of embelin at the same retention time as that of standard embelin. However, lot of variation in the percentage of embelin content was observed among the different samples. High percentage of embelin was observed in the samples collected from the Kerala (4.9%) followed by the Orrisa (4.6%). The percentage of embelin in Madhya Pradesh and Maharastra sample was 1.27 to 2.0%, respectively. The commercial samples collected from the local market showed low percentage of embelin (1.0 and1.05%). The HPLC procedure has given the reliable quantification of embelin from different samples, which can be used for analytical work and also for selection of embelin rich plant for commercial exploitation.

【 授权许可】

Unknown