Analysis of miRNAs Involved in Mouse Brain Damage upon Enterovirus 71 Infection

Frontiers in Cellular and Infection Microbiology

Liang, Yuan¹  Qiu, Shaofu¹  Liu, Nan¹  Yang, Chaojie¹  Wu, Fuli¹  Li, Hao¹  Liu, Hongbo¹  Liang, Beibei¹  Ma, Qiuxia¹  Yang, Xiaoxia¹  Sheng, Chunyu¹  Du, Xinying¹  Xie, Jing¹  Wang, Jinyan¹  Song, Hongbin¹  Jia, Leili¹  Li, Yongrui² 

[1] Center for Infectious Disease Control, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China;The Key Laboratory of Pharmacology and Molecular Biology, Medical College, Henan University of Science and Technology, Luoyang, China

关键词: Enterovirus 71 (EV71);    miRNA;    Brain;    Central Nervous System;    Inflammation;    neural system;    Encephalitis;   

DOI  :  10.3389/fcimb.2017.00133

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Enterovirus 71 (EV71) infects the central nervous system (CNS) and causes brainstem encephalitis in children. MiRNAs have been found to play various functions in EV71 infection in human cell lines. To identify potential miRNAs involved in the inflammatory injury in CNS, our study, for the first time, performed a miRNA microarray assay in vivo using EV71 infected mice brains. Twenty differentially expressed miRNAs were identified (four up- and sixteen down-regulated) and confirmed by qRT-PCR. The target genes of these miRNAs were analyzed using KEGG (Kyoto Encyclopaedia of Genes and Genomes) analysis, revealing that the miRNAs were mainly involved in the regulation of inflammation and neural system function. MiR-150-5p, -3082-5p, -3473a, -468-3p, -669n, -721, -709, and -5107-5p that regulate MAPK and chemokine signaling were all down-regulated, which might result in increased cytokine production. In addition, miR-3473a could also regulate focal adhesion and leukocyte trans-endothelial migration, suggesting a role in virus-induced blood-brain barrier disruption. The miRNAs and pathways identified in this study could help to understand the intricate interactions between EV71 and the brain injury, offering new insight for the future research of the molecular mechanism of EV71 induced brainstem encephalitis.

【 授权许可】

CC BY