Isolation of piperdine from Piper nigrum and its antiproliferative activity

Dynamic Chiropractic

关键词: Alkaloids;    piperine;    HPTLC;    antibacterial activity;    purification;    piperidine;    HEp2 cell lines.;   

DOI  :  10.5897/JMPR10.033

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Many plant derived molecules have shown a promising effect in therapeutics. Among the plants investigated to date, one showing enormous potential is the Piperaceae. Piperine is an alkaloid found naturally in plants belonging to the pyridine group of Piperaceae family, such asPipernigrumandPiperlongum. It is widely used in various herbal cough syrups and it is also used in anti inflammatory, anti malarial, anti leukemia treatment. So the present study was aimed to extract the phytochemical compounds in different solvent system inP.longum,P.nigrumandPipercubeba. In preliminary screening and confirmatory test it was identified as alkaloid. The methanol extract ofP. nigrum,P. longumand ethanol extract ofP. nigrumand ethyl acetate extract ofP. cubebashowed good result in TLC so these extracts were taken for HPTLC analysis. In HPTLC analysis ofP. nigrumsample showed six alkaloid bands, two alkaloid bands were similar to Piperine standard 1 and 2, the other alkaloid may be piperidine, piperettine and piperanine. InP. longumsample contain three alkaloid bands one band was similar to Piperine standard 1, the other may be piperlongumine and piperlonguminine and no alkaloid band was found inP. cubeba. The anti bacterial was tested against gram positive and negative organism using agar well diffusion method. High activity was found inP. nigrumethanol extract against the organismSalmonella typii. The alkaloid piperdine was purified by refluxion method to check the antitumour activity which shows 51.38% of inhibition at 5 µg/ml concentration that conforms the compound piperidine to be used as anticancer drug for further mechanistic works.

【 授权许可】

Unknown