Sinomenine suppresses expression of interleukin-1beta-induced matrix metalloproteinases in human osteoarthritic chondrocytes

Dynamic Chiropractic

关键词: Sinomenine;    interleukin-1;    osteoarthritis;    chondrocytes;    matrix metalloproteinases.;   

DOI  :  10.5897/JMPR10.251

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Sinomenine (SN) is an alkaloid extracted from the medicinal plant,Sinomenium acutumwhich has been utilized to treat rheumatoid arthritis (RA) in China for centuries. Previous studies showed that SN had anti-inflammatory and anti-rheumatic effects. The aim of the present study was to investigate the effect of SN on interleukin-1beta (IL-1beta)-induced expression of major cartilage damaging proteases in human osteoarthritic chondrocytes.Human osteoarthritic chondrocytes were obtained from the knee of patients undergoing total knee arthroplasty surgery due to OA.Humanosteoarthritic chondrocytes and SW1353 cells were plated inmonolayer culture.Cell viability was evaluated using MTT assay.SNwas added before stimulation with 10 ng/mL human recombinantIL-1beta.mRNA expression of MMPs was measured by RT-PCR. Protein expression was detected by Western blotting. SN suppressed IL-1beta-induced MMP-1, MMP-3, MMP-9, and MMP-13 mRNA and protein expression in SW1353 cells and human osteoarthritic chondrocytes. SN inhibited the catabolic effect of IL-1beta by interception of proteolytic enzymes expression. The cartilagecatabolic proteases inhibitory abilityand the well-known anti-inflammatory activity make SN a potentially novel therapeutic agent for OA.

【 授权许可】

Unknown