Antisickling and toxicological profiles of leaf and stem of Parquetina nigrescens L.

Dynamic Chiropractic

G. O. Gbenle¹  N. O. A. Imaga²  S. O. Edeoghon³  V. I. Okochi³  S.O. Adenekan⁴  M. O. Kehinde⁵ 

[1] College of Medicine of the University of Lagos,;Department of Biochemistry, Department of Medicine, College of Medicine of the University of Lagos,;of Medicine, Obstetrics and Gynecology, College of Medicine of the University of Lagos, P. M. B.12003, Idi-Araba, Lagos State, Nigeria.

关键词: Antisickling activity;    membrane integrity;    osmotic fragility;    Parquetinanigrescens;    sickle cell disease.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Folk medicine reportedly usesParquetinanigrescensL. (Asclepiadaceae) as a herbal remedy for the management of sickle cell anemia. This study was carried out to screen the leaves and stem ofP.nigrescensfor antisickling activity, erythrocyte membrane -stabilizing effects and any end organ toxicity. Percentage Reversal and Inhibition of Sickling parameters were analyzed on pre-sickled HbSSblood cell suspensions using sodium metabisulphite solution as inducer and 5 mg/ml parahydroxybenzoic acid and normal saline as positive and negative controls respectively. Effects of the plant extracts on the erythrocyte were assessed using osmotic fragility and the toxicity profile done via LD50and Sub-Acute toxicity studies on graded concentrations of extract. Results show thatP.nigrescenshas appreciable antisickling activity, has no toxic effect when administered at low concentrations and protects the integrity of the erythrocyte membrane as evidenced in the fragiliogram by the reduction in hemolysis of the Hbsscells.

【 授权许可】

Unknown