In vitro propagation of Clitoria ternatea L. : A rare medicinal plant

Dynamic Chiropractic

eya¹  Krishna P² 

[1] Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi-221005, U. P., India.;Department of Dravyaguna, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, U. P., India.

关键词: Clitoria ternatea;    aparajita;    axillary shoot proliferation.;   

DOI  :  10.5897/JMPR09.418

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

A rapid micro-propagation protocol was established for rare medicinal plantClitoria ternateaL. Multiple shoot formation was induced from shoot tip, node and cotyledonary node explants on Murashige and Skoog (MS) medium containing 2.0 mg/l BAP. Nodal explants gave the largest number (11.1) of shoots followed by cotyledonary node and shoot tip. MS medium containing 0.5 mg/l GA3was found most suitable for shoot elongation.Ex vitrorooting was achieved when the basal cut ends of elongated shoots were dipped in 250 mg/l IBA solution for half an hour followed by transplantation in plastic cups containing sterilized soilrite. The acclimatized plantlets were successfully established in the field with 90% survival rate. The plantlets are growing well without any phenotypic aberrations.

【 授权许可】

Unknown