Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts

Dynamic Chiropractic

Najla Gooda Sahib¹  Azizah Osman²  Nazamid Saari³  Mohd Sabri Pak-Dek⁴ 

[1] Agro-Biotechnology Institute, Ministry of Science, Technology and Innovation of Malaysia, Malaysia.;Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.;Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan.;Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

关键词: Morinda citrifolialeaf;    antioxidant extraction;    total phenolics;    ferric reducing potential;    2;    2-diphenyl-1-picrylhydrazyl (DPPH);    catechin;    high performance liquid chromatography (HPLC).;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The effect of four extraction techniques namely; solvent extraction (SE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and supercritical fluid extraction (SFE)on the phenolic components and antioxidant activity ofMorinda citrifolialeaf extracts was investigated.Total phenolics compounds (TPC) were quantified spectrophotometrically using Follin-Ciocalteu regent method, while the catechins were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). Antioxidant activity of the extracts was evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH) capacity and ferric ion reducing antioxidant potential (FRAP). Overall, MAE produced extract exhibited the maximum amount of TPC and catechin while UAE-extract had the highest antioxidant activity. It could be concluded that, even though MAE extract contained high TPC and catechin, the accelerated temperature used during this extraction technique might have attributed to reduce the antioxidant activity of this extract. Therefore, UAE can be recommended for recovery of potent natural antioxidant components fromM. citrifolialeaf offering better antioxidant activity.

【 授权许可】

Unknown