Ginsenoside Rg1 decrease expression of TNF- in rats with hyperlipidemia

Dynamic Chiropractic

关键词: Hyperlipidemia;    Ginsenoside Rg1;    tumor necrosis factor-alpha (TNF-α    );    reverse transcription-polymerase chain reaction (RT-PCR);    enzyme-linked immunosorbent assay (ELISA);    immunohistochemistry.;   

DOI  :  10.5897/JMPR11.1489

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Proinflammatory cytokines, such as IL-6 and tumor necrosis factor-alpha (TNF-alpha), are suggested to have an important role in the process ofhyperlipidemia. However, proinflammatory cytokines changes after using ginsenoside was little report. The aim of our study was to evaluate changes from tumor necrosis factor-alpha (TNF-α) ofhyperlipidemiarats after using ginsenoside Rg1 treatment by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry technology. The results demonstrated that TNF-α was significantly down-regulated in Rg1 treatment group in comparison to thehyperlipidemiagroup on mRNA level (P < 0.05), and Rg1 group and control group had no significantly difference on mRNA level (P < 0.05). On protein level, TNF-α was also highly decreased in Rg1 treatment group in comparison tohyperlipidemiagroup. The present study provides improvement in understanding the molecular pathogenic mechanism ofhyperlipidemiaand development ginsenoside Rg1 as effective treatment drugs.

【 授权许可】

Unknown