Study on the extraction process and tyrosinase inhibition property of cichoric acid in Echinacea purpurea L.

Dynamic Chiropractic

关键词: Echinacea purpureaL.;    cichoric acid;    extraction;    orthogonal design;    antimicrobial activity.;   

DOI  :  10.5897/JMPR11.1393

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Echinacea purpureahas become a commonly used herbal medicine because it contains various biologically activecompounds, such as cichoric acid. Extraction conditions of cichoric acid from the flowers ofE. purpureaL. were investigated through an orthogonal design of L9(34) assay. For purification, the extract was adsorbed on an AB-8 macroporous resin and eluted with 50% ethanol, then extracted with ethyl acetate four times, resulting in a residue containing 60.23% cichoric acid. Tyrosinase inhibition property of cichoric acid extracts was tested compared with arbutin by Spectrophotometer method. The extraction was optimized by adding 40% ethanol at a ratio of 1:15 (g/mL) to extract three times at 70C for 1 h. The cichoric acid extracts fromE. purpureawas found to have significant tyrosinase inhibition activity in broad range of concentration (≥10 mg/mL).

【 授权许可】

Unknown