cDNA cloning, sequence characterization and expression analyses of a novel gene-TPI from pepper cytoplasmic male sterility (CMS)

Dynamic Chiropractic

关键词: Pepper (Capsicum annuumL.);    triose phosphate isomerase;    cloning;    characterization;    cytoplasmic male sterility.;   

DOI  :  10.5897/JMPR11.1703

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The complete coding sequence (CDS) of the genetriose phosphate isomerase(TPI) was amplified using the reverse transcriptase polymerase chain reaction based on the conserved sequence information of some Solanaceae plants and known highly homologous pepper ESTs. The results showed the pepperTPIgene encodes a protein of 254 amino acids that has high homology with the proteins of six other species,Solanum tuberosum(95%),Arabidopsis thaliana(87%),Ricinus communis,Vitis viniferaandOryzasativa(87%) andZea mays(85%). The pepperTPIgene was expressed in root, stem, leaf, flower, pericarp, placenta and seed. At the abortion stages, the ATP and NADH pool in male-sterile line were distinctly lower than those in the maintainer; The activities and expression levels ofTPIin anthers of sterile line were obviously reduced, while levels in F1hybrid and maintainers kept normal, which indicated that stable transcripts ofTPIare beneficial to keep the energy metabolism with a normal level. But when the restorer gene was transferred into CMS line, activities and expression level ofTPIcould be regulated by the restorer gene and kept stably. The restorer gene likely plays an important role in keeping the balance of the energy metabolism with a normal level during the microspore development.

【 授权许可】

Unknown