Evaluation of spasmolytic and analgesic activity of ethanolic extract of Chenopodium album Linn and its fractions

Dynamic Chiropractic

NOOR JAHAN¹  Mansoor Ahmad²  Mehjabeen³  MUNIR Anwar⁴  Omair Anwar Mohiuddin⁵ 

[1] Department of Nuclear Medicine, Karachi Institute of Radio Therapy and Nuclear Medicine, Karachi, Pakistan.;Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, University of Karachi, Karachi-75270, Pakistan.;Department of Pharmacology, Federal Urdu University of Arts, Science and Technology, Karachi-75300, Pakistan.;Department of Pharmacology, Jinnah Postgraduate Medical Centre, Karachi, Pakistan.;Dow College of Pharmacy, Dow University of Health sciences, Karachi-74200, Pakistan.

关键词: Chenopodium albumLinn;    high performance liquid chromatography (HPLC);    Fourier transform infrared (FTIR);    Fourier transforms near infrared(FT-NIR) smooth muscles;    analgesic activity.;   

DOI  :  10.5897/JMPR12.412

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Chenopodium albumLinn is commonly consumed as vegetable and has been traditionally used for the treatment of hepatic disorders and inflammation.Standardization ofC. albumwas carried out by using ultraviolet (UV)-spectrophotometer, high performance liquid chromatography (HPLC),Fourier transforms near infrared (FT-NIR) andFourier transform infrared (FTIR).The plant was extracted in ethanol and fractionated in ethyl acetate, chloroform,n-butanol and water. The crude extract and its fractions were testedin vitroon intestinal smooth muscles of rabbit. The crude extract exhibited a dose-dependent increase in relaxation of smooth muscles, starting from 5 mg/ml and maximum effect was found at 20 mg/ml (92.86%). All the fractions were administered to rabbit’s intestine at 15 mg/ml dose. The ethyl acetate and chloroform fractions ofC. albumexhibited relaxation of the intestinal muscles (43.48 and 51.52%, respectively); whereas,n-butanol fraction ofC. albumproduced strong relaxant effect (91.18%). The contractile effect was only observed in aqueous fraction (29.41%). Overall, the activity produced byn-butanol fraction was found to be highly significant (by statistical analysis). Analgesic effect of the crude extract was carried out by tail flick method in mice. Significant analgesiceffect was observed at 500 mg/kg dose from 30 min up till 210 min.

【 授权许可】

Unknown