Sequencing of blaIMP-Carrying IncN2 Plasmids, and Comparative Genomics of IncN2 Plasmids Harboring Class 1 Integrons

Frontiers in Cellular and Infection Microbiology

Sun, Qiang¹  Jiang, Xiaoyuan¹  Zhou, Dongsheng¹  Chen, Shuiping²  Song, Yajun³  Yin, Xiuyun⁴  Feng, Jiao⁵  Yin, Zhe⁶  Wang, Jinglin⁶  Xu, Yuanhong⁶  Chen, Weijun⁶  Tong, Yigang⁶  Zhang, Defu⁶  Fang, Haihong⁷ 

[1] Anhui Medical University, Hefei, China;Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China;College of Food Science and Project Engineering, Bohai University, Jinzhou, China;Department of Clinical Laboratory, The 307th Hospital of the People'Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, China;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China;s Liberation Army, Beijing, China

关键词: IncN2 plasmids;    Class 1 Integron;    Transposon;    BlaIMP;    antimicrobial resistance;   

DOI  :  10.3389/fcimb.2017.00102

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

This work presents the complete nucleotide sequences of p0801-IMP from Klebsiella pneumoniae, p7121-IMP from K. oxytoca, and p17285-IMP from Citrobacter freundii, which are recovered from three different cases of nosocomial infection. These three plasmids represent the first fully sequenced blaIMP-carrying IncN2 plasmids. Further comparative genomics analysis of all the five integron-carrying IncN2 plasmids p0801-IMP, p7121-IMP, p17285-IMP, pJIE137, and p34983-59.134k indicates that they possess conserved IncN2 backbones with limited genetic variations with respect to gene content and organization. Four class 1 integrons (blaIMP-1-carrying In1223 in p0801-IMP/p7121-IMP, blaIMP-8-carrying In655 in p17285-IMP, In27 in pJIE137, and In1130 in p34983-59.134kb), two insertion sequence-based transposition units (ISEcp1-orfRA1-14 in p17285-IMP, and ISEcp1-blaCTX-M-62-Δorf477-orfRA1-14 in pJIE137), and a novel Tn1696-related transposon Tn6325 carrying In1130 in p34983-59.134kb are indentified in the plasmid accessory regions. In1223 and In655 represent ancestral Tn402-associated integrons, while In27 and In1130 belong to complex class 1 integrons. The relatively small IncN2 backbones are able to integrate different mobile elements which carry various resistance markers, promoting the accumulation and spread of antimicrobial resistance genes among enterobacterial species.

【 授权许可】

CC BY