Olea ferrugenia: A potential natural source of protection from oxidative stress

Dynamic Chiropractic

Muhammad Athar Abbasi¹  Sabahat Zahra Siddiqui²  Aziz-ur-Rehman³  Tauheeda Riaz⁴ 

[1] Department of Botany, Government College University, Lahore-54000, Pakistan.;Department of Chemistry, Government College University, Lahore-54000, Pakistan.;HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.

关键词: Olea ferrugeniaRoyale;    DPPH assay;    total antioxidant activity;    FRAP value;    total phenolics;    inhibition of lipid peroxidation (%).;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The methanolic extract ofOlea ferrugeniaRoyale was dissolved in distilled water and partitioned withn-hexane, chloroform, ethyl acetate andn-butanol successively. The phytochemical screening of all these organic fractions and remaining aqueous fraction was carried out and then the antioxidant potential of all studied fractions was evaluated by fourin vitromethods: DPPH free radical scavenging activity, total antioxidant activity, FRAP assay and ferric thiocyanate assay. Total phenolics of these organic fractions were also determined. All the fractions showed significant antioxidant potential. The results revealed that ethyl acetate soluble fraction showed highest value of percentage inhibition of DPPH radical (92.96% ± 0.13), with IC50value of (19.76 ± 0.12 µg/ml, relative to butylated hydroxytoluene (BHT), having IC50of 12.1 ± 0.92 µg/mL. This fraction also exhibited highest FRAP value (714.45 ± 2.61 µg of trolox equivalents), highest total phenolic contents (416.4.59 ± 7.73 mg of gallic acid equivalents), as well as highest value of inhibition of lipid peroxidation (57.51% ± 1.56) when compared with other fractions. However, the chloroform soluble fraction showed highest total antioxidant activity (0.9101 ± 0.03) when compared with other fractions.

【 授权许可】

Unknown