Improved in vitro culture and micropropagation of different Melissa officinalis L. genotypes

Dynamic Chiropractic

H. Meftahizade¹  M. Lofti²  B. Naseri³  H. Moradkhani⁴ 

[1] ACECR Medicinal Plants Research Center, Ilam Iran.;Department of Horticultural, Aboureihan Campus, University of Tehran, Iran.;Faculty of Islamic Azad University, Ilam, Iran.;Student of Agricultural Biotechnology, Armenia.

关键词: Melissa officinalis;    regeneration;    micropropagation;    Iran.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Melissa officinalis L.is widely cultivated in the world and Iran as well, due to its useful application in medicine. In order to establish a stable and high frequency of regeneration system with 4 landrace collected from different climate in Iran, major parameters such as regeneration rate, rooting percentage, shooting and proliferation rate were investigated. Statistical analysis of results showed that BAP in combination with NAA had the highest regeneration in shoot tips explants. NAA in combination with IAA and Kinetin had the best response to callus induction. Also 1 mgl/l NAA had the higher response to rooting than other’s used auxins. Explants derived from Hamedan landrace had the highest potential for regeneration. This is the first report on high frequencyin vitroregeneration in four diverse landraces ofM. officinalisselected from various climates regions in Iran.

【 授权许可】

Unknown