Molecular cloning and analysis of a novel HMW-GS gene Glu1-St1.5 from Elymus sibiricus in Qinghai-Tibetan Plateau

Scientific Research and Essays

LIU Jin¹ 

关键词: Glu1-1St1.5;    HMW-GS;     ;    Elymus sibiricus.;   

DOI  :  10.5897/SRE11.1220

学科分类：社会科学、人文和艺术（综合）

来源: Academic Journals

【 摘 要 】

In the present study, a HMW-GS alleleGlu1-St1.5was isolated and characterized fromElymus sibiricus. Nucleotide sequence analysis indicated that the St1.5 shared the highest similarity with St1.3 fromElymus canadensis, but the “TA” insertion mutation at 15 to 16 bp in signal peptide sequence made it an inactive gene, because three premature stop codons were generated by “TA” insertion in signal peptide region, N-terminal region and C-terminal region, respectively. To identify the type of St1.5, the inserted “TA” was eliminated factitiously and the deduced amino acid sequence was analyzed, the result showed that St1.5 was a y-type HMW-GS pseudo gene. Phylogenetic tree demonstrated that St1.5 had the closest relationship to the St1 allele ofPseudoroegneria stipifolia, which indicated that St1.5 was a novel gene of St Genome. These results provide insights into the evolutionary biology ofGlu1-St1.5and other HMW-GS genes.

【 授权许可】

CC BY