Multiple antibiotic susceptibility of polyphosphate kinase mutants ( ppk1 and ppk2 ) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis

Biological research: BR

Catalina Bravo-Toncio¹  Javiera Ortiz-Severí²  Macarena Varas²  n²  Nicolá² 

[1] Bacterial Communication Laboratory, Department of Biology, Faculty of Science, University of Chile, Santiago, Chile;Systems Microbiology Laboratory, Department of Biology, Faculty of Science, University of Chile, Santiago, Chile

关键词: Antibiotic susceptibility;    Polyphosphate kinase;    Pseudomonas;    Phenome;    Phenotypic microarrays;    Multidrug resistance;   

DOI  :  10.1186/s40659-015-0012-0

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence. By using Phenotypic Microarrays (PM) we analyzed near 2000 phenotypes of P. aeruginosa PAO1 polyP kinase mutants (ppk1 and ppk2). We found that both ppk mutants shared most of the phenotypic changes and interestingly many of them related to susceptibility toward numerous and different type of antibiotics such as Ciprofloxacin, Chloramphenicol and Rifampicin. Combining the fact that ppk1 mutants have reduced virulence and are more susceptible to antibiotics, polyP synthesis and particularly PPK1, is a good target for the design of molecules with anti-virulence and anti-persistence properties.

【 授权许可】

CC BY