A Bacterial Acetyltransferase Destroys Plant Microtubule Networks and Blocks Secretion

PLoS Pathogens

Van Quach¹  Brenden Hurley¹  Carmen Yea¹  David S. Guttman²  Darrell Desveaux²  Verena Bartetzko³  Jennifer D. Lewis³  Amy Huei-Yi Lee³  Corinna Felsensteiner³  Yulu C. Liu³  Wenzislava Ckurshumova³  Stephane Angers⁴  Pauline W. Wang⁴  Frederik Börnke⁵  Andrew Wilde⁵ 

[1] Centre for the Analysis of Genome Evolution & Function, University of Toronto, Toronto, Ontario, Canada;Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada;Department of Cell & Systems Biology, University of Toronto, Toronto, Ontario, Canada;Institut für Biologie, Lehrstuhl für Biochemie, Friedrich Alexander Universität Erlangen-Nürnberg, Germany;Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada

关键词: Microtubules;    Tubulins;    Arabidopsis thaliana;    Pseudomonas syringae;    Plant bacterial pathogens;    Eukaryota;    Secretion;    Plant pathogens;   

DOI  :  10.1371/journal.ppat.1002523

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The eukaryotic cytoskeleton is essential for structural support and intracellular transport, and is therefore a common target of animal pathogens. However, no phytopathogenic effector has yet been demonstrated to specifically target the plant cytoskeleton. Here we show that the Pseudomonas syringae type III secreted effector HopZ1a interacts with tubulin and polymerized microtubules. We demonstrate that HopZ1a is an acetyltransferase activated by the eukaryotic co-factor phytic acid. Activated HopZ1a acetylates itself and tubulin. The conserved autoacetylation site of the YopJ / HopZ superfamily, K289, plays a critical role in both the avirulence and virulence function of HopZ1a. Furthermore, HopZ1a requires its acetyltransferase activity to cause a dramatic decrease in Arabidopsis thaliana microtubule networks, disrupt the plant secretory pathway and suppress cell wall-mediated defense. Together, this study supports the hypothesis that HopZ1a promotes virulence through cytoskeletal and secretory disruption.

【 授权许可】

CC BY