PLoS Pathogens | |
Atypical/Nor98 Scrapie Infectivity in Sheep Peripheral Tissues | |
Stéphanie Simon1  Nathalie Morel1  Annick Le Dur2  Vincent Beringue2  Hubert Laude2  Leonor Orge3  Sylvie L. Benestad4  Caroline Lacroux5  Fabien Corbière5  François Schelcher5  Claire Litaise5  Olivier Andréoletti5  Séverine Lugan5  Pierrette Costes5  Hugh Simmons6  | |
[1] CEA, Service de Pharmacologie et d'Immunoanalyse, IBiTec-S, DSV, CEA/Saclay, Gif sur Yvette cedex, France;INRA UR892, Virologie et Immunologie Moléculaires, INRA, F-78350 Jouy-en-Josas, France;Laboratório Nacional de Investigação Veterinária, Lisboa, Portugal;National Veterinary Institute, Oslo, Norway;UMR INRA ENVT 1225, Interactions Hôtes Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France;VLA Weybridge, ASU, Addlestone, Surrey, United Kingdom | |
关键词: Scrapie; Sheep; Animal prion diseases; Central nervous system; Brainstem; Muscle tissue; Ruminants; Cerebral cortex; | |
DOI : 10.1371/journal.ppat.1001285 | |
学科分类:生物科学(综合) | |
来源: Public Library of Science | |
【 摘 要 】
Atypical/Nor98 scrapie was first identified in 1998 in Norway. It is now considered as a worldwide disease of small ruminants and currently represents a significant part of the detected transmissible spongiform encephalopathies (TSE) cases in Europe. Atypical/Nor98 scrapie cases were reported in ARR/ARR sheep, which are highly resistant to BSE and other small ruminants TSE agents. The biology and pathogenesis of the Atypical/Nor98 scrapie agent in its natural host is still poorly understood. However, based on the absence of detectable abnormal PrP in peripheral tissues of affected individuals, human and animal exposure risk to this specific TSE agent has been considered low. In this study we demonstrate that infectivity can accumulate, even if no abnormal PrP is detectable, in lymphoid tissues, nerves, and muscles from natural and/or experimental Atypical/Nor98 scrapie cases. Evidence is provided that, in comparison to other TSE agents, samples containing Atypical/Nor98 scrapie infectivity could remain PrPSc negative. This feature will impact detection of Atypical/Nor98 scrapie cases in the field, and highlights the need to review current evaluations of the disease prevalence and potential transmissibility. Finally, an estimate is made of the infectivity loads accumulating in peripheral tissues in both Atypical/Nor98 and classical scrapie cases that currently enter the food chain. The results obtained indicate that dietary exposure risk to small ruminants TSE agents may be higher than commonly believed.
【 授权许可】
CC BY
【 预 览 】
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