Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad

Journal of venomous animals and toxins

Sandro Rostelato-Ferreira¹  a Rodrigues-Simioni¹  Gildo B Leite²  riston A Dal Belo³  Chá⁴  Lé⁵  Stephen Hyslop⁶ 

[1] Departamento de Farmacologia, Faculdade de CiêLaboratódicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, Campinas, Brasil;ncias Méo Gabriel, Brazil;rio de Neurobiologia e Toxinologia, (LANETOX), Universidade Federal do Pampa, (UNIPAMPA), Sã

关键词: Neurotransmitter release;    Ouabain;    Presynaptic;    Rhinella schneideri;    Toad venom;   

DOI  :  10.1186/1678-9199-20-30

学科分类：药理学

来源: BioMed Central

【 摘 要 】

Rhinella schneideri, previously known as Bufo paracnemis, is a common toad in many regions of Brazil. Its venom exerts important cardiovascular effects on humans and other animals. Although this toad venom has been the subject of intense investigations, little is known about its neuromuscular activity. The neurotoxicity of a methanolic extract of R. schneideri venom was tested on mouse phrenic nerve-diaphragm (PND) preparations mounted for conventional twitch tension recording – in response to indirect stimulation – and for electrophysiological measurements. Venom extract (50 μg/mL) increased the muscle twitch tension in PND preparations but did not significantly alter the resting membrane potential values. Electrophysiological evaluations showed that the extract (50 μg/mL) significantly augmented the frequency of miniature end-plate potential (from 38 ± 3.5 to 88 ± 15 after 60 minutes; n = 5; p 

【 授权许可】

CC BY