Human Papillomavirus Type 16 Entry: Retrograde Cell Surface Transport along Actin-Rich Protrusions

PLoS Pathogens

Minna-Liisa Rajamäki¹  Mario Schelhaas²  Helge Ewers²  Ari Helenius²  Patricia M. Day³  John T. Schiller³ 

[1] Department of Applied Biology, University of Helsinki, Helsinki, Finland;Institute of Biochemistry, ETH Zurich, Zurich, Switzerland;Laboratory of Cellular Oncology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of Maryland

关键词: HPV-16;    Actins;    HeLa cells;    Confocal microscopy;    Myosins;    Actin polymerization;    Cell membranes;    Human papillomavirus infection;   

DOI  :  10.1371/journal.ppat.1000148

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The lateral mobility of individual, incoming human papillomavirus type 16 pseudoviruses (PsV) bound to live HeLa cells was studied by single particle tracking using fluorescence video microscopy. The trajectories were computationally analyzed in terms of diffusion rate and mode of motion as described by the moment scaling spectrum. Four distinct modes of mobility were seen: confined movement in small zones (30–60 nm in diameter), confined movement with a slow drift, fast random motion with transient confinement, and linear, directed movement for long distances. The directed movement was most prominent on actin-rich cell protrusions such as filopodia or retraction fibres, where the rate was similar to that measured for actin retrograde flow. It was, moreover, sensitive to perturbants of actin retrograde flow such as cytochalasin D, jasplakinolide, and blebbistatin. We found that transport along actin protrusions significantly enhanced HPV-16 infection in sparse tissue culture, cells suggesting a role for in vivo infection of basal keratinocytes during wound healing.

【 授权许可】

CC BY