Study of the influence of solvent, carbohydrate and fatty acid in the enzymatic synthesis of sugar esters by lipases

Quimica nova

Paula, Ariela V. de¹  Castro, Heizir F. de¹  Barboza, Jayne C. de Souza¹ 

[1] Faculdade de Engenharia Química de Lorena, Lorena

关键词: lipase;    carbohydrate;    esters.;   

DOI  :  10.1590/S0100-40422005000500011

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The aim of thiswork was to gain knowledge of enzymatic processes for the synthesis fatty acidesters of sugar, with the objective to develop an enzymatic process for thepreparation of non-toxic biodegradable surface-active agents derived entirelyfrom renewable resources. A wide range of data were collected for reaction conditionsinvolving different sugars (glucose, fructose and sucrose), fatty acids (oleic,palmitic, lauric), solvents (hexane, heptane and t-butanol) and different sourcesof lipases in both free and immobilized forms. As a solvent t-butanol providedthe best conditions to create a catalytic liquid phase in which the reactionoccurs. Sugars were preferentially esterified in the following order: fructose> glucose > sucrose, depending on the enzyme preparation. For fructoseno influence was found concerning de acyl donor and similar rates were achievedfor all tested fatty acids. Ester synthesis was maximized for substrates containingfructose, lauric or oleic acids, t-butanol and lipase from porcine pancreasimmobilized on polysiloxane-polyvinyl alcohol particles. Under such conditionsmolar conversions were higher than 50%.

【 授权许可】

CC BY