Hemolytic activity of venom from crown-of-thorns starfish Acanthaster planci spines

Journal of venomous animals and toxins

Chi-Chiu Lee¹  Deng-Fwu Hwang¹  Hernyi Justin Hsieh²  Wann-Sheng Tsai³ 

[1] Department of Food Science and Center of Excellence for Marine Bioenvironment and Biotechnology, National Taiwan Ocean University, Taiwan, ROC;Department of Health and Nutrition Biotechnology, Asia University, Taiwan, ROC;Penghu Marine Biology Research Center, Council of Agriculture, Fisheries Research Institute, Taiwan, Magong, Penghu, ROC

关键词: Crown-of-thorns starfish;    Spine;    Venoms;    Hemolysis;   

DOI  :  10.1186/1678-9199-19-22

学科分类：药理学

来源: BioMed Central

【 摘 要 】

The crown-of-thorns starfish Acanthaster planci is a venomous species from Taiwan whose venom provokes strong hemolytic activity. To understand the hemolytic properties of A. planci venom, samples were collected from A. planci spines in the Penghu Islands, dialyzed with distilled water, and lyophilized into A. planci spine venom (ASV) powder. Both crude venom and ASV cause 50% hemolysis at a concentration of 20 μg/mL. The highest hemolytic activity of ASV was measured at pH 7.0-7.4; ASV-dependent hemolysis was sharply reduced when the pH was lower than 3 or greater than 8. There was almost no hemolytic activity when the Cu2+ concentration was increased to 10 mM. Furthermore, incubation at 100°C for 30 to 60 minutes sharply decreased the hemolytic activity of ASV. After treatment with the protease α-chymotrypsin, the glycoside hydrolase cellulase, and the membrane component cholesterin, the hemolytic activity of ASV was significantly inhibited. The results of this study provide fundamental information about A. planci spine venom. The hemolytic activity was affected by pH, temperature, metal ions, EDTA, cholesterin, proteases, and glycoside hydrolases. ASV hemolysis was inhibited by Cu2+, cholesterin, α-chymotrypsin, and cellulose, factors that might prevent the hemolytic activity of venom and provide the medical treatment for sting.

【 授权许可】

CC BY