Enzymatic modification on rice flour seeking the production of resitant starch

Quimica nova

Severo, Márcio Garcia¹  Moraes, Kelly de¹  Ruiz, Walter Augusto¹ 

[1] Universidade Federal do Rio Grande, Rio Grande, Brasil

关键词: amyloglucosidase;    modifying native starches;    debranching.;   

DOI  :  10.1590/S0100-40422010000200021

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The aim of thiswork was to study the enzymatic modification on rice flour using lipase pancreaticand amyloglucosidase to obtain resistant starch. For this, Response SurfaceMethodology (RSM) was used to determine the best operating conditions for eachenzyme. For lypase pancreatic, the highest value for resistant starch (45%)was achieved within 2 h reaction at pH 7 using an enzyme/substrate ratio of4% (w/w) and Dp= 100/200 tyler. For amyloglucosidase, optima conditions correspondedto an enzyme/substrate ratio of 0,006 mL/g and Dp= 100/200 tyler at 45 ºC, yielding57% of resistant starch in 2 h reaction. These results show the potential ofusing both enzymes to modified rice flour, increasing the resistant starch inabout 5.7 folds in relation to the flour without treatment (resistant starch=10.6%).

【 授权许可】

CC BY