New Perspectives on Host-Parasite Interplay by Comparative Transcriptomic and Proteomic Analyses of Schistosoma japonicum

PLoS Pathogens

Ming Chi¹  Xin-Rong Wang¹  Xin Zhang¹  Feng Liu¹  Wei Hu¹  Qing Yan¹  Huai-Dong Song¹  Chun-Liang Xue¹  Zhi-Qin Wang¹  Shu-Jian Cui¹  Ze-Guang Han¹  Paul J Brindley²  Donald P McManus³  Ju-Jun Wang⁴  Sheng-Yue Wang⁴  Xiang-Lin Zhang⁴  Zhu Chen⁴  Zheng Feng⁵  Jiong Lu⁵  Peng-Yuan Yang⁶  Xue-Nian Xu⁷ 

[1] Chinese National Human Genome Center at Shanghai, Shanghai, China;Department of Parasitology, Shanghai Second Medical University, Shanghai, China;Department of Tropical Medicine, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America;National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China;Proteomic Center and Department of Chemistry, Fudan University, Shanghai, China;Queensland Institute of Medical Research and Australian Center for International Health and Nutrition, Brisbane, Queensland, Australia;State Key Laboratory of Medical Genomics, Shanghai Second Medical University, Shanghai, China

关键词: Schistosoma japonicum;    Expressed sequence tags;    Tegument proteins;    Transcriptome analysis;    Proteomic databases;    Schistosoma;    Schistosoma mansoni;    Motor proteins;   

DOI  :  10.1371/journal.ppat.0020029

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs. Comparative analysis of these transcriptomic and proteomic data, the latter including 3,260 proteins with putative identities, revealed differential expression of genes among the various developmental stages and sexes of S. japonicum and localization of putative secretory and membrane antigens, enzymes, and other gene products on the adult tegument and eggshell, many of which displayed genetic polymorphisms. Numerous S. japonicum genes exhibited high levels of identity with those of their mammalian hosts, whereas many others appeared to be conserved only across the genus Schistosoma or Phylum Platyhelminthes. These findings are expected to provide new insights into the pathophysiology of schistosomiasis and for the development of improved interventions for disease control and will facilitate a more fundamental understanding of schistosome biology, evolution, and the host-parasite interplay.

【 授权许可】

CC BY