Scientific Research and Essays | |
Expressing of rice ribosome inactivating protein as tool for treatment of cancer cells | |
Ali Salehzadeh1  | |
关键词: Ribosome-inactivating proteins; Pichia pastoris; N-glycosidases.; | |
DOI : 10.5897/SRE11.1073 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: Academic Journals | |
【 摘 要 】
Ribosome-inactivating proteins (RIPs) are toxic proteins synthesized by many plants and some bacteria, that specifically depurinate the 28S RNA and thus interrupt protein translation. RIPs hold broad interest because of their potential use as plant defense factors against pathogens and cancer cells. The most promising way to exploit plant RIPs as weapons against cancer cells is either by designing molecules in which the toxic domains of RIPs are linked to selective tumor targeting domains or directly delivered as suicide genes for cancer gene therapy. In this research the sequence of rice RIP gene was synthesized in PUC57 plasmid. The gene was cloned in pPICZαA vector and expressed inPichia pastorisKM71H (muts) strain. The expected protein which had an apparent molecular mass of 34 kDa was detected by SDS-PAGE analysis and confirmed by Western blot.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO201902018419918ZK.pdf | 293KB | download |