RNAi Screen in Drosophila Cells Reveals the Involvement of the Tom Complex in Chlamydia Infection

PLoS Pathogens

Alice Dautry-Varsat¹  Marc Pypaert²  Isabelle Derré³  Hervé Agaisse⁴ 

[1] Center for Cell and Molecular Imaging, Yale University School of Medicine, New Haven, Connecticut, United States of America;Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, United States of America;Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven Connecticut, United States of America;Unité de Biologie des Interactions Cellulaires, Institut Pasteur, Paris, France

关键词: Chlamydia infection;    Drosophila melanogaster;    Chlamydia trachomatis;    Mitochondria;    Chlamydia;    Small interfering RNAs;    Host cells;    Cell differentiation;   

DOI  :  10.1371/journal.ppat.0030155

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Chlamydia spp. are intracellular obligate bacterial pathogens that infect a wide range of host cells. Here, we show that C. caviae enters, replicates, and performs a complete developmental cycle in Drosophila SL2 cells. Using this model system, we have performed a genome-wide RNA interference screen and identified 54 factors that, when depleted, inhibit C. caviae infection. By testing the effect of each candidate's knock down on L. monocytogenes infection, we have identified 31 candidates presumably specific of C. caviae infection. We found factors expected to have an effect on Chlamydia infection, such as heparansulfate glycosaminoglycans and actin and microtubule remodeling factors. We also identified factors that were not previously described as involved in Chlamydia infection. For instance, we identified members of the Tim-Tom complex, a multiprotein complex involved in the recognition and import of nuclear-encoded proteins to the mitochondria, as required for C. caviae infection of Drosophila cells. Finally, we confirmed that depletion of either Tom40 or Tom22 also reduced C. caviae infection in mammalian cells. However, C. trachomatis infection was not affected, suggesting that the mechanism involved is C. caviae specific.

【 授权许可】

CC BY