In Vitro Derived Dendritic Cells trans-Infect CD4 T Cells Primarily with Surface-Bound HIV-1 Virions

PLoS Pathogens

Warner C Greene¹  Marielle Cavrois²  Jason Neidleman²  Jason F Kreisberg² 

[1] Department of Medicine, University of California San Francisco, San Francisco, California, United States of America;Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, California, United States of America

关键词: Virions;    T cells;    HIV-1;    Cell fusion;    T helper cells;    Immunostaining;    Synapses;    Dendritic cells;   

DOI  :  10.1371/journal.ppat.0030004

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

In the prevailing model of HIV-1 trans-infection, dendritic cells (DCs) capture and internalize intact virions and transfer these virions to interacting T cells at the virological synapse. Here, we show that HIV-1 virions transmitted in trans from in vitro derived DCs to T cells principally originate from the surface of DCs. Selective neutralization of surface-bound virions abrogated trans-infection by monocyte-derived DCs and CD34-derived Langerhans cells. Under conditions mimicking antigen recognition by the interacting T cells, most transferred virions still derived from the cell surface, although a few were transferred from an internal compartment. Our findings suggest that attachment inhibitors could neutralize trans-infection of T cells by DCs in vivo.

【 授权许可】

CC BY